Download the single-particle cryo-electron microscopy datasheet to review hardware configurations optimized for high-resolution structural analysis. This CSV file details the technical specifications of our screening and data collection microscopes, including electron energy, Cs values, and advanced detector/filter combinations like the Falcon 4i with Selectris and Gatan K3.

Download Datasheet

Access to the instrumentation is restricted to active users affiliated with a member institution [ß hyperlink to the list of institutions]. To register as a user, please follow the steps below:

1. Create an Account: Create a MEMC account for yourself with the following form: MEMC Account Registration. Note: You must create an account from a member institution email address.
    
2. Register Your Project: After creating a new account, register your project by clicking "Add a New Project."
    
3. Attend New User Orientation: Orientation is offered the first Tuesday of every month, from 9:30 a.m. to 12:00 p.m. Please email Dianne Carpen at help@nysbc.org to inquire about future orientation session dates.
    
4. Schedule a Project Meeting: Arrange a meeting with the MEMC staff by emailing memc@nysbc.org and notify your institution’s representative.
   • Non-Krios instrumentation may be booked using our scheduler.
   • If a Krios microscope is needed, please contact your institution’s representative to secure a Krios session.

Access through NCCAT: The NIH National Center for CryoEM Access and Training, housed at SEMC, is open to researchers nationwide regardless of member-institution affiliation, free of charge. NCCAT provides access to state-of-the-art instrumentation, including Titan Krios microscopes with direct detectors, specimen-preparation robots, and screening microscopes, backed by direct staff support and cross-training. Time is awarded through an open, competitive proposal process with rolling quarterly cycles, submitted via NCCAT Proposal Central (nccat.nysbc.org/proposalcentral). For help choosing the right access route, contact nccatuseroffice@nysbc.org.

Proposals access categories:

• GUP1 / BAG: Krios single-particle data collection for experienced users with pre-screened grids
   
• GUP2: staff-assisted sample preparation and screening (Chameleon)
   
• GUP3 (GPS): grid preparation and screening for groups with limited local access, on-site visits, or remote Glacios screening
   
• TP1: embedded researcher cross-training toward independent cryo-EM practice
   
• TP2: facility-manager “train-the-trainer” immersion
   
• Rapid Access (subject to capacity)
   
• NCCAT is one of three National Cryo-EM Service Centers, alongside the Pacific Northwest Center for Cryo-EM (PNCC) and the Stanford-SLAC Cryo-EM Center (S²C²). All three centers operate independently, with distinct access categories and independent review cycles. Applicants are welcome to apply to multiple centers simultaneously. For more information, please visit https://www.cryoemcenters.org/.
   
• Training
  • We strongly recommend that collaborators and users undergo training on the specific instrumentation required for their project. A tailored training plan with clear dates and goals will be designed during your project meeting. The hands-on training is conducted one-on-one with a staff member during sessions that actively advance the project.
     
  • Collaborators are categorized into three levels during the training pipeline: “Requires Assistance,” “Supervision Needed,” or “Independent User”. To be certified as an “Independent User,” two separate staff members must verify your proficiency on the system, which includes earning the instrument’s US CryoEM Center Merit Badge where applicable.
     
  • Learn more about our Education & Outreach

SEMC Acknowledgement: Any use of the resources at SEMC requires acknowledgement of use of the resources in publications as follows:

“Some of this work was performed at the Simons Electron Microscopy Center at the New York Structural Biology Center, with major support from the Simons Foundation (SF349247).”

NCCAT Acknowledgement: Any use of the resources at NCCAT requires acknowledgment of use of the resources in presentations and publications as follows:

“Some of this work was performed at the National Center for CryoEM Access and Training (NCCAT) and the Simons Electron Microscopy Center located at the New York Structural Biology Center, supported by the NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program (U24 GM129539, and NIGMS R24 GM154192) and by grants from the Simons Foundation (SF349247) and NY State Assembly.”