Electron Microscopy
Access:
Seeing biology at molecular resolution—from purified complexes to whole cells
NYSBC operates one of the world’s most comprehensive platforms for cryo-electron microscopy. It encompasses four main equipment ecosystems: transmission electron microscopes optimized for single-particle imaging and cellular tomography; cryo-focused ion beam systems for in situ sample preparation; integrated light microscopy for correlative workflows; and a complete set of sample preparation and supporting technologies. Together, these capabilities enable researchers to visualize molecular structure across the full range of biological complexity—from purified assemblies at near-atomic resolution to intact cellular environments in their native state. With all of these tools under one management structure, expertise and data workflows are shared. Users can thus access complementary capabilities seamlessly, moving from initial sample screening to structure determination and through to manuscript-ready analysis.
The facility’s approach integrates cutting-edge instrumentation with the development of methods that reduce barriers to use. Whether scientists are imaging a well-characterized complex or exploring new territory, NYSBC’s staff work alongside them to optimize sample preparation, data collection, and analysis. Member institutions access the facility directly; national researchers apply through NCCAT or NCITU.
Single Particle Cryo-Electron Microscopy
Cryo-Electron Tomography
Cryo-FIB (Focused Ion Beam)
Correlative Light and Electron Microscopy (CLEM)
Equipment
Gatan Solarus
Refeyn Mass Photometer
SPT Chameleon
Vitribot
Leica EM GP2
Leica EM GP
Orbitrap Exploris 480
PRIMO micropatterning system
Leica Stellaris 8
Zeiss LSM 900 with Airyscan 780
Arctis
Hydra
Aquilos2
Hitachi 7800
Tundra
Glacios Electron Microscope
Krios Electron Microscope
Publications
CryoET imaging reveals that mitotic spindles are locally organized by microtubule density and Eg5 activity
Nature Communications
The Signaling Pathway of a Bacterial Two-Component System Determined by cryo-EM
Quantifying RNA Folding Equilibria from Unclassified Cryo-EM Particle Images
Overcoming air–water interface-induced artifacts in cryo-EM with protein nanocrates
Nature Methods, Vol. 23, Issue 9, Pages 1798-1804
A bacteriophytochrome Pr/Pfr heterodimer studied through single-particle time-resolved cryo-electron microscopy
Communications Chemistry, Vol. 9, Issue 1
Characterization and modulation of human insulin degrading enzyme conformational dynamics to control enzyme activity
eLife, Vol. 14
Characterization and modulation of human insulin degrading enzyme conformational dynamics to control enzyme activity
eLife, Vol. 14
SCM-1/SCAMP Maintains Microdomain Boundaries and Cargo Sorting within the Endosomal System
Simultaneous single-cell profiling of the transcriptome and proteome
Molecular insight into microtubule nucleation by the XMAP215/γ-TuRC module
Nature Communications, Vol. 17, Issue 1
Eg5 activity and density-driven bundling organize the human metaphase mitotic spindle independently of spindle bipolarity
CryoEM Merit Badges as a Training Framework to Expand Your Structural Biology Experimental Toolkit
JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 302, Issue 5, Pages 111492