Download the correlative microscopy datasheet for comprehensive technical specifications of SEMC's instrumentation. This resource provides detailed system parameters—including electron energy, Cs values, and detector types—across our Krios and Glacios microscopes, DualBeam FIB-SEM systems, and sample preparation equipment.

Download Datasheet

• MEMC (collaborative access) with user resources

• Access to the cryo-CLEM instrumentation is restricted to active users affiliated with a member institution [ß hyperlink to the list of institutions]. If you meet these criteria and are interested in collaborating with our staff, please send an email to help[at]nysbc.org with the subject line “MEMC cryo-CLEM Information Request - <Your Name>”. In the body of your email, please provide a short description of the project that you are interested in pursuing. We will follow up via email to schedule an initial project meeting.

• NCITU (in situ) with user resources 
  • Access through the National Center for In Situ Ultramicroscopy (NCITU) capabilities, including cryo-CLEM support, is managed through NCITU policies and the joint tomography center application process. Users should first meet with the in situ team to define the workflow, determine whether plunge freezing or HPF is appropriate for the specimen, and identify whether the project is best supported as a service, a training opportunity, or a combination of both. NCITU staff provides guidance and hands-on support as needed, with training options designed to build the skills required for independent cryo-ET sample preparation where appropriate.
• Training
  • We strongly recommend that collaborators undergo training on the specific instrumentation required for their project. A tailored training plan with clear dates and goals will be designed during your project meeting. The hands-on training is conducted one-on-one with a staff member during sessions that actively advance the project.
  •  Collaborators are categorized into three levels during the training pipeline: “Requires Assistance,” “Supervision Needed,” or “Independent User.” To be certified as an “Independent User,” two separate staff members must verify your proficiency on the system, which includes earning the instrument’s US CryoEM Center Merit Badge [ß hyperlink to the cryoEM merit badge] where applicable.

Any use of the resources at SEMC requires acknowledgement of use of the resources in publications as follows:

“Some of this work was performed at the Simons Electron Microscopy Center at the New York Structural Biology Center, with major support from the Simons Foundation (SF349247).”

NCITU Acknowledgement: 

The National Center for In-situ Tomographic Ultramicroscopy (NCITU) is one of four service centers that are part of the NIH Common Fund’s Transformative High Resolution Cryoelectron Microscopy (CryoEM) Program and funded under NIH Grant U24GM139171.

The National Network for CryoET will be established with four service centers, including one service center that will also serve as the central network hub. The network hub will be located at the University of Wisconsin–Madison and led by Dr. Elizabeth Wright. The network service centers will be located at the New York Structural Biology Center (Dr. Alex de Marco, contact), SLAC National Accelerator Laboratory–Stanford University (Dr. Wah Chiu), and University of Colorado Boulder (Dr. Andreas Hoenger, contact). The service centers will specialize in cryoET specimen preparation. The hub will perform cryoET data collection for all the user laboratories served by the network, in addition to its service center functions.

Any use of the resources at NCITU requires acknowledgment of use of the resources in presentations and publications as follows:

Standard Acknowledgement:

“Some of this work was performed at the National Center for In-Situ Tomographic Ultramicroscopy (NCITU) and the Simons Electron Microscopy Center located at the New York Structural Biology Center, supported by the NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program (U24 GM129539,) and by grants from the Simons Foundation (SF349247) and NY State Assembly.”