Download the cryo-FIB datasheet for complete technical specifications of SEMC’s focused ion beam milling instrumentation. This document details the configurations of our DualBeam systems—including the Aquilos 2, Hydra, and Arctis—highlighting FIB sources (LMIS and Plasma) and integrated fluorescence light microscopy (iFLM) detectors used for precise lamella preparation.

Download Datasheet

• MEMC (Arctis/collaborative access) with user resources
   

• Access to the Arctis cryo-FIB microscope is collaborative and restricted to active users affiliated with a member institution. If you meet these criteria and are interested in collaborating with our staff, please send an email to help[at]nysbc.org with the subject line “MEMC Arctis Information Request - <Your Name>”. In the body of your email, please provide a short description of the project that you are interested in pursuing. We will follow up via email to schedule an initial project meeting.

• Access to the Aquilos is available through NCITU, the National Center for In-situ Tomographic Ultramicroscopy. Prospective users should begin with a project meeting with the in situ team to discuss the biological question, assess feasibility, and determine the appropriate workflow before submitting through the joint tomography center portal. Depending on project goals, NCITU can provide either staff-supported service or hands-on training toward independent use, following NCITU policies for proposal review, scheduling, safety, and instrument access.

  • The National Center for In-situ Tomographic Ultramicroscopy (NCITU) is one 4 service centers that are part of the NIH Common Fund’s Transformative High Resolution Cryoelectron Microscopy (CryoEM) Program. NCITU is dedicated to developing and providing access to both standard and advanced methods for in-situ cryoET specimen preparation. Access to the center is managed by a central Network Coordinating Group (NCG) designed to ensure fair and equal access to a nationwide user base. For more information, please visit https://www.cryoetportal.org/   
     

Training

•  We strongly recommend that collaborators undergo training on the specific instrumentation required for their project. A tailored training plan with clear dates and goals will be designed during your project meeting. The hands-on training is conducted one-on-one with a staff member during sessions that actively advance the project.
   

• Collaborators are categorized into three levels during the training pipeline: “Requires Assistance,” “Supervision Needed,” or “Independent User.” To be certified as an “Independent User,” two separate staff members must verify your proficiency on the system, which includes earning the instrument’s US CryoEM Center Merit Badge where applicable.

SEMC Acknowledgement: Any use of the resources at SEMC requires acknowledgement of use of the resources in publications as follows:

“Some of this work was performed at the Simons Electron Microscopy Center at the New York Structural Biology Center, with major support from the Simons Foundation (SF349247).”

NCITU Acknowledgement: 

The National Center for In-situ Tomographic Ultramicroscopy (NCITU) is one of four service centers that are part of the NIH Common Fund’s Transformative High Resolution Cryoelectron Microscopy (CryoEM) Program and funded under NIH Grant U24GM139171.

The National Network for CryoET will be established with four service centers, including one service center that will also serve as the central network hub. The network hub will be located at the University of Wisconsin–Madison and led by Dr. Elizabeth Wright. The network service centers will be located at the New York Structural Biology Center (Dr. Bridget Carragher, contact), SLAC National Accelerator Laboratory–Stanford University (Dr. Wah Chiu), and University of Colorado Boulder (Dr. Andreas Hoenger, contact). The service centers will specialize in cryoET specimen preparation. The hub will perform cryoET data collection for all the user laboratories served by the network, in addition to its service center functions.

Standard Acknowledgement: Any use of the resources at NCITU requires acknowledgment of use of the resources in presentations and publications as follows:

“Some of this work was performed at the National Center for In-Situ Tomographic Ultramicroscopy (NCITU) and the Simons Electron Microscopy Center located at the New York Structural Biology Center, supported by the NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program (U24 GM129539,) and by grants from the Simons Foundation (SF349247) and NY State Assembly.”