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Biography

I am a scientist at SEMC speciallizing in cryoEM data collection and processing. My goal is to provide the biomedical research community with access to the expertise and resources available at SEMC, thereby expanding our understanding of both molecular structures and cellular structures. My scientific interest is focused on technology development aimed to improving the efficiency of data collection and processing.

Publications

A bacteriophytochrome Pr/Pfr heterodimer studied through single-particle time-resolved cryo-electron microscopy

Prabin Karki, William C. Budell, Rohit Kannachel, David Menendez, Carolina Hernandez, Joshua H. Mendez, Shemiran Kanou, Manjeet Singh, Chavdar Slavov, Peter Schwander, Tek Narsingh Malla, Emina A. Stojković, Marius Schmidt

Communications Chemistry, Vol. 9, Issue 1

2026 June

Characterization and modulation of human insulin degrading enzyme conformational dynamics to control enzyme activity

Jordan M Mancl, Wenguang G Liang, Nicholas L Bayhi, Hui Wei, William C Budell, Joshua H Mendez, Tobin R Sosnick, Bridget Carragher, Clinton S Potter, Wei-Jen Tang

eLife, Vol. 14

2026 June

Characterization and modulation of human insulin degrading enzyme conformational dynamics to control enzyme activity

Jordan M Mancl, Wenguang G Liang, Nicholas L Bayhi, Hui Wei, William C Budell, Joshua H Mendez, Tobin R Sosnick, Bridget Carragher, Clinton S Potter, Wei-Jen Tang

eLife, Vol. 14

2026 June

A bacteriophytochrome Pr/Pfr heterodimer as obtained by single-ms TR cryo-EM

Marius Schmidt, Prabin Karki, William Budell, Rohit Kannachel, David Menendez, Carolina Hernandez, Joshua Mendez, Shemiran Kanou, Manjeet Singh, Chavdar Slavov, Peter Schwander, Tek Malla, Emina Stojković

2025 November

Structures of myxobacterial phytochrome revealed by cryo-EM using the Spotiton technique and with x-ray crystallography

Prabin Karki, David Menendez, William Budell, Shishir Dangi, Carolina Hernandez, Joshua Mendez, Srinivasan Muniyappan, Shibom Basu, Peter Schwander, Tek N. Malla, Emina A. Stojković, Marius Schmidt

Structural Dynamics, Vol. 12, Issue 3

2025 May

Structure of dimerized assimilatory NADPH-dependent sulfite reductase reveals the minimal interface for diflavin reductase binding

Behrouz Ghazi Esfahani, Nidhi Walia, Kasahun Neselu, Yashika Garg, Mahira Aragon, Isabel Askenasy, Hui Alex Wei, Joshua H. Mendez, M. Elizabeth Stroupe

Nature Communications, Vol. 16, Issue 1

2025 March

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Ruth M. Saecker, Andreas U. Mueller, Brandon Malone, James Chen, William C. Budell, Venkata P. Dandey, Kashyap Maruthi, Joshua H. Mendez, Nina Molina, Edward T. Eng, Laura Y. Yen, Clinton S. Potter, Bridget Carragher, Seth A. Darst

Nature Structural & Molecular Biology, Vol. 31, Issue 11, Pages 1778-1788

2024 November

Photoreception and signaling in bacterial phytochrome revealed by single-particle cryo-EM

Tek Narsingh Malla, Carolina Hernandez, Srinivasan Muniyappan, David Menendez, Dorina Bizhga, Joshua H. Mendez, Peter Schwander, Emina A. Stojković, Marius Schmidt

Science Advances, Vol. 10, Issue 32

2024 August

Structure of dimerized assimilatory NADPH-dependent sulfite reductase reveals the minimal interface for diflavin reductase binding

M. Elizabeth Stroupe, Behrouz Ghazi Esfahani, Nidhi Walia, Kasahun Neselu, Mahira Aragon, Isabel Askenasy, Hui Wei, Joshua Mendez

2024 July

Structure of dimerized assimilatory NADPH-dependent sulfite reductase reveals the minimal interface for diflavin reductase binding

Behrouz Ghazi Esfahani, Nidhi Walia, Kasahun Neselu, Yashika Garg, Mahira Aragon, Isabel Askenasy, Hui Alex Wei, Joshua H. Mendez, M. Elizabeth Stroupe

2024 June

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Ruth M. Saecker, Andreas U. Mueller, Brandon Malone, James Chen, William C. Budell, Venkata P. Dandey, Kashyap Maruthi, Joshua H. Mendez, Nina Molina, Edward T. Eng, Laura Y. Yen, Clinton S. Potter, Bridget Carragher, Seth A. Darst

2024 March

Automated pipelines for rapid evaluation during cryoEM data acquisition

Joshua H. Mendez, Eugene Y.D. Chua, Mohammadreza Paraan, Clinton S. Potter, Bridget Carragher

Current Opinion in Structural Biology, Vol. 83, Pages 102729

2023 December

Smart Leginon enables cryoEM screening automation across multiple grids

Anjelique Sawh-Gopal, Aygul Ishemgulova, Eugene Y.D. Chua, Mahira F. Aragon, Joshua H. Mendez, Edward T. Eng, Alex J. Noble

2023 July

Structural basis for substrate selection by the SARS-CoV-2 replicase

Brandon F. Malone, Jason K. Perry, Paul Dominic B. Olinares, Hery W. Lee, James Chen, Todd C. Appleby, Joy Y. Feng, John P. Bilello, Honkit Ng, Johanna Sotiris, Mark Ebrahim, Eugene Y. D. Chua, Joshua H. Mendez, Ed T. Eng, Robert Landick, Matthias Götte, Brian T. Chait, Elizabeth A. Campbell, Seth A. Darst

Nature, Vol. 614, Issue 7949, Pages 781-787

2023 February

Characterizing the resolution and throughput of the Apollo direct electron detector

Ruizhi Peng, Xiaofeng Fu, Joshua H. Mendez, Peter S. Randolph, Benjamin E. Bammes, Scott M. Stagg

Journal of Structural Biology: X, Vol. 7, Pages 100080

2023 January

Fully automated multi-grid cryoEM screening using Smart Leginon

Anchi Cheng, Paul T. Kim, Huihui Kuang, Joshua H. Mendez, Eugene Y. D. Chua, Kashyap Maruthi, Hui Wei, Anjelique Sawh, Mahira F. Aragon, Viacheslav Serbynovskyi, Kasahun Neselu, Edward T. Eng, Clinton S. Potter, Bridget Carragher, Tristan Bepler, Alex J. Noble

IUCrJ, Vol. 10, Issue 1, Pages 77-89

2023 January

Smart Leginon: Fully Automated Cryo-EM Grid Screening for CryoEM using Leginon and Ptolemy

Huihui Kuang, Paul Kim, Anchi Cheng, Kashyap Maruthi, Eugene YD Chua, Joshua H Mendez, Hui Wei, Edward T Eng, Viacheslav Serbynovskyi, Clinton S Potter, Bridget Carragher, Alex J Noble, Tristan Bepler

Microscopy and Microanalysis, Vol. 28, Issue S1, Pages 1266-1268

2022 August

Evaluating the chameleon Sample Preparation Device: Case Studies

Mahira Aragon, Hui Wei, Eugene YD Chua, Joshua H Mendez, Bridget Carragher, Clinton S Potter

Microscopy and Microanalysis, Vol. 28, Issue S1, Pages 1202-1204

2022 August