Biography

As a Senior Scientist at NYSBC I focus on the application of electron microscopy to biomedical research by providing expertise and resources for understanding both molecular and cellular structures. I have extensive experience designing, renovating, operating, and managing cryoEM facilities. As a manager of one the an NIH sponsored cryoEM service centers, my mission is to lower the barriers of access to the cryoEM technology and cross-train researchers to have accelerated impact at their home institutions.

Publications

CryoEM Merit Badges as a Training Framework to Expand Your Structural Biology Experimental Toolkit

Christina Zimanyi, Mahira Aragon, Eugene YD. Chua, Dianne Carpen, Cathleen Castello, Elina Kopylov, Alex de Marco, Jeffrey Kieft, Edward Eng

JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 302, Issue 5, Pages 111492

2026 January

Using national center cryo-EM merit badges for standardization of best practices and modular training frameworks

BIOPHYSICAL JOURNAL

2026 January

Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci

William S. Henriques, Jarrett Bowman, Laina N. Hall, Colin C. Gauvin, Hui Wei, Huihui Kuang, Christina M. Zimanyi, Edward T. Eng, Andrew Santiago-Frangos, Blake Wiedenheft

2025 June

Applications of visualization technology in the structural sciences

Edward T. Eng, Nichole R. Valdez

Structural Dynamics, Vol. 12, Issue 3

2025 May

Architecture and activation of single-pass transmembrane receptor guanylyl cyclase

Shian Liu, Alexander M. Payne, Jinan Wang, Lan Zhu, Navid Paknejad, Edward T. Eng, Wei Liu, Yinglong Miao, Richard K. Hite, Xin-Yun Huang

Nature Structural & Molecular Biology, Vol. 32, Issue 3, Pages 469-478

2025 March

Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data

Carissa Chestnut, Jake D. Johnston, Marcus Velazquez, Kasahun Neselu, Edward T. Eng

Journal of Visualized Experiments, Issue 215

2025 January

Editorial: Breakthroughs in Cryo-EM with machine learning and artificial intelligence

Edward T. Eng, Sonya M. Hanson, Ruben Sanchez-Garcia

FRONTIERS IN MOLECULAR BIOSCIENCES, Vol. 12

2025 January

Growing the structural sciences community through a framework of service and engagement

BIOPHYSICAL JOURNAL

2025 January

Structures reveal how the Cast-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci

William S. Henriques, Jarrett Bowman, Laina N. Hall, Colin C. Gauvin, Hui Wei, Huihui Kuang, Christina M. Zimanyi, Edward T. Eng, Andrew Santiago-Frangos, Blake Wiedenheft

STRUCTURE, Vol. 33, Issue 12, Pages 2058-2070.e6

2025 January

NCCAT: Providing access to instrumentation and training to lower the barriers of entry for labs to utilize cryoEM

Christina Zimanyi, Mahira Aragon, Eugene Chua, Aaron Owji, Elina Kopylov, Dianne Carpen, Edward Eng, Jeffrey Kieft, Alex de Marco

JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 301, Issue 5, Pages 108585

2025 January

The 2024 New York City Integrative Structural Biology Symposium

Pilar Cossio, Edward T. Eng

Trends in Pharmacological Sciences, Vol. 46, Issue 1, Pages 1-8

2025 January

Multi-species cryoEM calibration and workflow verification standard

Daija Bobe, Mykhailo Kopylov, Jessalyn Miller, Aaron P. Owji, Edward T. Eng

Acta Crystallographica Section F Structural Biology Communications, Vol. 80, Issue 11, Pages 320-327

2024 November

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Ruth M. Saecker, Andreas U. Mueller, Brandon Malone, James Chen, William C. Budell, Venkata P. Dandey, Kashyap Maruthi, Joshua H. Mendez, Nina Molina, Edward T. Eng, Laura Y. Yen, Clinton S. Potter, Bridget Carragher, Seth A. Darst

Nature Structural & Molecular Biology, Vol. 31, Issue 11, Pages 1778-1788

2024 November

Multi-species cryoEM calibration and workflow verification standard

Daija Bobe, Mykhailo Kopylov, Jessalyn Miller, Aaron P. Owji, Edward T. Eng

2024 August

Applying 3D ED/MicroED workflows toward the next frontiers

Mahira Aragon, Sarah E. J. Bowman, Chun-Hsing Chen, M. Jason de la Cruz, Daniel A. Decato, Edward T. Eng, Kristen M. Flatt, Sahil Gulati, Yuchen Li, Charles J. Lomba, Brandon Mercado, Jessalyn Miller, Lukáš Palatinus, William J. Rice, David Waterman, Christina M. Zimanyi

Acta Crystallographica Section C Structural Chemistry, Vol. 80, Issue 6, Pages 179-189

2024 June

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

bioRxiv

2024 March

CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization

Viacheslav Serbynovskyi, Jing Wang, Eugene YD Chua, Aygul Ishemgulova, Lambertus M. Alink, William C. Budell, Jake D. Johnston, Charlie Dubbeldam, Fabio A. Gonzalez, Sharon Rozovsky, Edward T. Eng, Alex de Marco, Alex J. Noble

bioRxiv

2024 January

Establishing a cross-facility-accepted training curriculum through a system of shared standards

BIOPHYSICAL JOURNAL

2024 January