Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci

William S. Henriques, Jarrett Bowman, Laina N. Hall, Colin C. Gauvin, Hui Wei, Huihui Kuang, Christina M. Zimanyi, Edward T. Eng, Andrew Santiago-Frangos, Blake Wiedenheft

Applications of visualization technology in the structural sciences

Edward T. Eng, Nichole R. Valdez

Structural Dynamics, Vol. 12, Issue 3

Architecture and activation of single-pass transmembrane receptor guanylyl cyclase

Shian Liu, Alexander M. Payne, Jinan Wang, Lan Zhu, Navid Paknejad, Edward T. Eng, Wei Liu, Yinglong Miao, Richard K. Hite, Xin-Yun Huang

Nature Structural & Molecular Biology, Vol. 32, Issue 3, Pages 469-478

The 2024 New York City Integrative Structural Biology Symposium

Pilar Cossio, Edward T. Eng

Trends in Pharmacological Sciences, Vol. 46, Issue 1, Pages 1-8

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Ruth M. Saecker, Andreas U. Mueller, Brandon Malone, James Chen, William C. Budell, Venkata P. Dandey, Kashyap Maruthi, Joshua H. Mendez, Nina Molina, Edward T. Eng, Laura Y. Yen, Clinton S. Potter, Bridget Carragher, Seth A. Darst

Nature Structural & Molecular Biology, Vol. 31, Issue 11, Pages 1778-1788

Multi-species cryoEM calibration and workflow verification standard

Daija Bobe, Mykhailo Kopylov, Jessalyn Miller, Aaron P. Owji, Edward T. Eng

Acta Crystallographica Section F Structural Biology Communications, Vol. 80, Issue 11, Pages 320-327

Multi-species cryoEM calibration and workflow verification standard

Daija Bobe, Mykhailo Kopylov, Jessalyn Miller, Aaron P. Owji, Edward T. Eng

Applying 3D ED/MicroED workflows toward the next frontiers

Mahira Aragon, Sarah E. J. Bowman, Chun-Hsing Chen, M. Jason de la Cruz, Daniel A. Decato, Edward T. Eng, Kristen M. Flatt, Sahil Gulati, Yuchen Li, Charles J. Lomba, Brandon Mercado, Jessalyn Miller, Lukáš Palatinus, William J. Rice, David Waterman, Christina M. Zimanyi

Acta Crystallographica Section C Structural Chemistry, Vol. 80, Issue 6, Pages 179-189

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Ruth M. Saecker, Andreas U. Mueller, Brandon Malone, James Chen, William C. Budell, Venkata P. Dandey, Kashyap Maruthi, Joshua H. Mendez, Nina Molina, Edward T. Eng, Laura Y. Yen, Clinton S. Potter, Bridget Carragher, Seth A. Darst

CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization

Viacheslav Serbynovskyi, Jing Wang, Eugene YD Chua, Aygul Ishemgulova, Lambertus M. Alink, William C. Budell, Jake D. Johnston, Charlie Dubbeldam, Fabio A. Gonzalez, Sharon Rozovsky, Edward T. Eng, Alex de Marco, Alex J. Noble

bioRxiv

Promiscuous G-protein activation by the calcium-sensing receptor

Hao Zuo, Jinseo Park, Aurel Frangaj, Jianxiang Ye, Guanqi Lu, Jamie J. Manning, Wesley B. Asher, Zhengyuan Lu, Guo-bin Hu, Liguo Wang, Joshua Mendez, Edward Eng, Zhening Zhang, Xin Lin, Robert Grassucci, Wayne A. Hendrickson, Oliver B. Clarke, Jonathan A. Javitch, Arthur D. Conigrave, Qing R. Fan

Nature, Vol. 629, Issue 8011, Pages 481-488

Cryo-Electron Microscopy Screening Automation across Multiple Grids using Smart Leginon.

Anjelique Sawh-Gopal, Aygul Ishemgulova, Eugene Y. D. Chua, Mahira F. Aragon, Joshua H. Mendez, Edward T. Eng, Alex J. Noble

Journal of visualized experiments : JoVE, Issue 202

Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method

M. Jason de la Cruz, Edward T. Eng

Structure, Vol. 31, Issue 12, Pages 1487-1498

Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays

Andrew Santiago-Frangos, William S. Henriques, Tanner Wiegand, Colin C. Gauvin, Murat Buyukyoruk, Ava B. Graham, Royce A. Wilkinson, Lenny Triem, Kasahun Neselu, Edward T. Eng, Gabriel C. Lander, Blake Wiedenheft

Nature Structural & Molecular Biology, Vol. 30, Issue 11, Pages 1675-1685

Smart Leginon enables cryoEM screening automation across multiple grids

Anjelique Sawh-Gopal, Aygul Ishemgulova, Eugene Y.D. Chua, Mahira F. Aragon, Joshua H. Mendez, Edward T. Eng, Alex J. Noble

Protein-mediated folding of the genome is essential for site-specific integration of foreign DNA into CRISPR loci

Andrew Santiago-Frangos, William Henriques, Tanner Wiegand, Colin Gauvin, Murat Buyukyoruk, Kasahun Neselu, Edward Eng, Gabriel Lander, Royce Wilkinson, Ava Graham, Blake Wiedenheft

Protein-mediated genome folding allosterically enhances site-specific integration of foreign DNA into CRISPRs

Andrew Santiago-Frangos, William S. Henriques, Tanner Wiegand, Colin C. Gauvin, Murat Buyukyoruk, Ava B. Graham, Royce A. Wilkinson, Lenny Triem, Kasahun Neselu, Edward T. Eng, Gabriel C. Lander, Blake Wiedenheft

Structural basis for substrate selection by the SARS-CoV-2 replicase

Brandon F. Malone, Jason K. Perry, Paul Dominic B. Olinares, Hery W. Lee, James Chen, Todd C. Appleby, Joy Y. Feng, John P. Bilello, Honkit Ng, Johanna Sotiris, Mark Ebrahim, Eugene Y. D. Chua, Joshua H. Mendez, Ed T. Eng, Robert Landick, Matthias Götte, Brian T. Chait, Elizabeth A. Campbell, Seth A. Darst

Nature, Vol. 614, Issue 7949, Pages 781-787