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Biography

I am a staff scientist at SEMC with a focus on cryoEM technology development, and grid preparation and screening.

Publications

Stabilization of the hexasome intermediate during histone exchange by yeast SWR1 complex.

Adam S.B. Jalal, Paul Girvan, Eugene Y.D. Chua, Lexin Liu, Shijie Wang, Elizabeth A. McCormack, Michael T. Skehan, Carol L. Knight, David S. Rueda, Dale B. Wigley

Molecular cell, Vol. 84, Issue 20, Pages 3871-3884.e9

2024 August

Square beams for optimal tiling in transmission electron microscopy

Eugene Y. D. Chua, Lambertus M. Alink, Mykhailo Kopylov, Jake D. Johnston, Fabian Eisenstein, Alex de Marco

Nature Methods, Vol. 21, Issue 4, Pages 562-565

2024 April

Automated pipelines for rapid evaluation during cryoEM data acquisition

Joshua H. Mendez, Eugene Y.D. Chua, Mohammadreza Paraan, Clinton S. Potter, Bridget Carragher

Current Opinion in Structural Biology, Vol. 83, Pages 102729

2023 December

Cryo-Electron Microscopy Screening Automation across Multiple Grids using Smart Leginon.

Anjelique Sawh-Gopal, Aygul Ishemgulova, Eugene Y. D. Chua, Mahira F. Aragon, Joshua H. Mendez, Edward T. Eng, Alex J. Noble

Journal of visualized experiments : JoVE, Issue 202

2023 December

Structural basis for substrate selection by the SARS-CoV-2 replicase

Brandon F. Malone, Jason K. Perry, Paul Dominic B. Olinares, Hery W. Lee, James Chen, Todd C. Appleby, Joy Y. Feng, John P. Bilello, Honkit Ng, Johanna Sotiris, Mark Ebrahim, Eugene Y. D. Chua, Joshua H. Mendez, Ed T. Eng, Robert Landick, Matthias Götte, Brian T. Chait, Elizabeth A. Campbell, Seth A. Darst

Nature, Vol. 614, Issue 7949, Pages 781-787

2023 February

Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 Å resolution with cryo-EM.

Robert C Monsen, Eugene Y D Chua, Jesse B Hopkins, Jonathan B Chaires, John O Trent

Nucleic acids research, Vol. 51, Issue 4, Pages 1943-1959

2023 February

Measuring the effects of ice thickness on resolution in single particle cryo-EM.

Kasahun Neselu, Bing Wang, William J. Rice, Clinton S. Potter, Bridget Carragher, Eugene Y.D. Chua

Journal of structural biology: X, Vol. 7, Pages 100085

2023 January

Fully automated multi-grid cryoEM screening using Smart Leginon

Anchi Cheng, Paul T. Kim, Huihui Kuang, Joshua H. Mendez, Eugene Y. D. Chua, Kashyap Maruthi, Hui Wei, Anjelique Sawh, Mahira F. Aragon, Viacheslav Serbynovskyi, Kasahun Neselu, Edward T. Eng, Clinton S. Potter, Bridget Carragher, Tristan Bepler, Alex J. Noble

IUCrJ, Vol. 10, Issue 1, Pages 77-89

2023 January

Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy

Eugene Y.D. Chua, Joshua H. Mendez, Micah Rapp, Serban L. Ilca, Yong Zi Tan, Kashyap Maruthi, Huihui Kuang, Christina M. Zimanyi, Anchi Cheng, Edward T. Eng, Alex J. Noble, Clinton S. Potter, Bridget Carragher

Annual Review of Biochemistry, Vol. 91, Issue 1, Pages 1-32

2022 June

Vitrocam: A simple low cost Vitrobot camera for assessing grid quality

Eugene Y.D. Chua, Viacheslav Serbynovskyi, Robert Gheorghita, Lambertus M. Alink, Daniel Podolsky, Clinton S. Potter, Bridget Carragher

2022 June

Structure and dynamics of the yeast SWR1-nucleosome complex

Oliver Willhoft, Mohamed Ghoneim, Chia-Liang Lin, Eugene Y. D. Chua, Martin Wilkinson, Yuriy Chaban, Rafael Ayala, Elizabeth A. McCormack, Lorraine Ocloo, David S. Rueda, Dale B. Wigley

Science, Vol. 362, Issue 6411

2018 October

Advances in phase plate cryo-EM imaging of DNA and nucleosomes.

Eugene Y. D. Chua, Sara Sandin

Nucleus (Austin, Tex.), Vol. 8, Issue 3, Pages 275-278

2017 May

3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM.

Eugene Y.D. Chua, Vinod K. Vogirala, Oviya Inian, Andrew S.W. Wong, Lars Nordenskiöld, Juergen M. Plitzko, Radostin Danev, Sara Sandin

Nucleic acids research, Vol. 44, Issue 17, Pages 8013-8019

2016 September

Stereochemical control of nucleosome targeting by platinum-intercalator antitumor agents.

E. Y. D. Chua, G. E. Davey, C. F. Chin, P. Droge, W. H. Ang, C. A. Davey

Nucleic acids research, Vol. 43, Issue 11, Pages 5284-5296

2015 June

The mechanics behind DNA sequence-dependent properties of the nucleosome.

Eugene Y. D. Chua, Dileep Vasudevan, Gabriela E. Davey, Bin Wu, Curt A. Davey

Nucleic acids research, Vol. 40, Issue 13, Pages 6338-6352

2012 July

Crystal structures of nucleosome core particles containing the '601' strong positioning sequence.

Dileep Vasudevan, Eugene Y.D. Chua, Curt A. Davey

Journal of molecular biology, Vol. 403, Issue 1, Pages 1-10

2010 October