Imaging large fields-of-view at high resolution in cryo-ET with square beam montaging

Eugene Y.D. Chua, Hamidreza Rahmani, James Zhen, Fabian Eisenstein, Yong Hyun Song, Jake D. Johnston, Hanyu Wang, Lambertus M. Alink, Mykhailo Kopylov, Chi-Min Ho, Danielle A Grotjahn, Alex de Marco

Electrostatic Self-Assembly of Protein Nanoparticles from Intrinsically Disordered Polypeptide and Globular Protein Pairs

So Yeon Ahn, Megan S.K. Jen, Dayoung Gloria Lee, Jake D Johnston, Jane Liao, Eun Sol Kim, Ying Tong Yue, Shana Elbaum, Oleg Gang, Allie C Obermeyer

Overcoming air-water interface-induced artifacts in Cryo-EM with protein nanocrates

Matthew C Jenkins, Daija Bobe, Jake D Johnston, Jonah Cheung, Akira Karasawa, Christina M Zimanyi, Ömer Dermanci, M.G. Finn, Alex de Marco, Mykhailo Kopylov

CryoDRGN-AI: neural ab initio reconstruction of challenging cryo-EM and cryo-ET datasets

Axel Levy, Rishwanth Raghu, J. Ryan Feathers, Michal Grzadkowski, Frédéric Poitevin, Jake D. Johnston, Francesca Vallese, Oliver Biggs Clarke, Gordon Wetzstein, Ellen D. Zhong

Nature Methods, Vol. 22, Issue 7, Pages 1486-1494

Cationic amino acid identity and net charge influence condensate properties in E. coli

Aaron K. Kidane, Jacob R. Rosenfeld, Jake D. Johnston, Charlie Dubbeldam, Mohammadreza Paraan, Allie C. Obermeyer

Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data

Carissa Chestnut, Jake D. Johnston, Marcus Velazquez, Kasahun Neselu, Edward T. Eng

Journal of Visualized Experiments, Issue 215

Accurate and fast segmentation of filaments and membranes in micrographs and tomograms with TARDIS

Robert Kiewisz, Gunar Fabig, Will Conway, Jake Johnston, Victor A. Kostyuchenko, Aaron Tan, Cyril Bařinka, Oliver Clarke, Magdalena Magaj, Hossein Yazdkhasti, Francesca Vallese, Shee-Mei Lok, Stefanie Redemann, Thomas Müller-Reichert, Tristan Bepler

CryoDRGN-ET: deep reconstructing generative networks for visualizing dynamic biomolecules inside cells

Ramya Rangan, Ryan Feathers, Sagar Khavnekar, Adam Lerer, Jake D. Johnston, Ron Kelley, Martin Obr, Abhay Kotecha, Ellen D. Zhong

Nature Methods, Vol. 21, Issue 8, Pages 1537-1545

Endoplasmic reticulum–plasma membrane contact gradients direct cell migration

Bo Gong, Jake D. Johnston, Alexander Thiemicke, Alex de Marco, Tobias Meyer

Nature, Vol. 631, Issue 8020, Pages 415-423

Square beams for optimal tiling in transmission electron microscopy

Eugene Y. D. Chua, Lambertus M. Alink, Mykhailo Kopylov, Jake D. Johnston, Fabian Eisenstein, Alex de Marco

Nature Methods, Vol. 21, Issue 4, Pages 562-565

CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization

Viacheslav Serbynovskyi, Jing Wang, Eugene YD Chua, Aygul Ishemgulova, Lambertus M. Alink, William C. Budell, Jake D. Johnston, Charlie Dubbeldam, Fabio A. Gonzalez, Sharon Rozovsky, Edward T. Eng, Alex de Marco, Alex J. Noble

bioRxiv

Mitochondrial protein FKBP8 captures PDZD8 to form mitochondria-ER contacts

Koki Nakamura, Saeko Aoyama-Ishiwatari, Takahiro Nagao, Mohammadreza Paaran, Christopher J. Obara, Yui Sakurai-Saito, Jake Johnston, Yudan Du, Shogo Suga, Masafumi Tsuboi, Makoto Nakakido, Kouhei Tsumoto, Yusuke Kishi, Yukiko Gotoh, Chulhwan Kwak, Hyun-Woo Rhee, Jeong Kon Seo, Hidetaka Kosako, Clint Potter, Bridget Carragher, Jennifer Lippincott-Schwartz, Franck Polleux, Yusuke Hirabayashi

Modern Tools for <i>In-situ</i> Tomography

Mykhailo Kopylov, Daija Bobe, Jake D Johnston, Reza M Paraan

Microscopy and Microanalysis, Vol. 29, Issue Supplement_1, Pages 954-955

Recent advances in infectious disease research using cryo-electron tomography

Daniel Asarnow, Vada A. Becker, Daija Bobe, Charlie Dubbledam, Jake D. Johnston, Mykhailo Kopylov, Nathalie R. Lavoie, Qiuye Li, Jacob M. Mattingly, Joshua H. Mendez, Mohammadreza Paraan, Jack Turner, Viraj Upadhye, Richard M. Walsh, Meghna Gupta, Edward T. Eng

Frontiers in Molecular Biosciences, Vol. 10

Architecture of the human erythrocyte ankyrin-1 complex

Francesca Vallese, Kookjoo Kim, Laura Y. Yen, Jake D. Johnston, Alex J. Noble, Tito Calì, Oliver Biggs Clarke

Nature Structural &amp; Molecular Biology, Vol. 29, Issue 7, Pages 706-718

In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method

Oleg Klykov, Daija Bobe, Mohammadreza Paraan, Jake Johnston, Clinton Potter, Bridget Carragher, Mykhailo Kopylov, Alex Noble

BIO-PROTOCOL, Vol. 12, Issue 21

Structure of the G protein chaperone and guanine nucleotide exchange factor Ric-8A bound to Gαi1

Levi J. McClelland, Kaiming Zhang, Tung-Chung Mou, Jake Johnston, Cindee Yates-Hansen, Shanshan Li, Celestine J. Thomas, Tzanko I. Doukov, Sarah Triest, Alexandre Wohlkonig, Gregory G. Tall, Jan Steyaert, Wah Chiu, Stephen R. Sprang

Nature Communications, Vol. 11, Issue 1

Crystal and cryo-EM structures of the cytosolic G protein alpha chaperone and guanine nucleotide exchange factor Ric-8A bound to Gαi1

Levi J. McClelland, Kaiming Zhang, Tung-Chung Mou, Jake Johnston, Cindee Yates-Hansen, Shanshan Li, Celestine J. Thomas, Tzanko I. Doukov, Sarah Triest, Alexandre Wohlkonig, Gregory G. Tall, Jan Steyaert, Wah Chiu, Stephen R. Sprang