Biography

Anthony Sica (she/they) works on the development advanced cryogenic light microscopy methods to be introduced into the cryo CLEM workflow. Currently, they are utilizing time-correlated single photon correlation methods to categorize and develop a library of fluorescent markers commonly used in biology and observing how spectral and time-dependent photophysical processes change at lower temperatures. Alongside this, they are also developing a fast throughput method to measure the crystallinity of ice within cryogenic samples. Allowing a quick and robust method to assess the quality of freezing the sample early within the workflow will save money and time in the cryogenic workflow. In their spare time they like to climb, gather friends for board games, play music, and spend time with their partner and two cats.

Publications

Optical interference for the guidance of cryogenic focused ion beam milling beyond the axial diffraction limit

Anthony V. Sica, Magda Zaoralová, Cali Antolini, Daan B. Boltje, Judit J. Penzes, Lilyana M. Malmqvist, Grant J. Jensen, Jason T. Kaelber, Peter D. Dahlberg

Nature Communications, Vol. 17, Issue 1

2026 January

Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells

Jue Wang, Anthony V. Sica, Grant J. Jensen, Peter D. Dahlberg

Journal of Visualized Experiments, Issue 224

2025 October

Optical Interference for the Guidance of Cryogenic Focused Ion Beam Milling Beyond the Axial Diffraction Limit

Anthony V. Sica, Magda Zaoralová, Cali Antolini, Daan B. Boltje, Judit J. Penzes, Lilyana M. Malmqvist, Grant Jensen, Jason T. Kaelber, Peter Dahlberg

2024 November

De novo design of proteins housing excitonically coupled chlorophyll special pairs

Nathan M. Ennist, Shunzhi Wang, Madison A. Kennedy, Mariano Curti, George A. Sutherland, Cvetelin Vasilev, Rachel L. Redler, Valentin Maffeis, Saeed Shareef, Anthony V. Sica, Ash Sueh Hua, Arundhati P. Deshmukh, Adam P. Moyer, Derrick R. Hicks, Avi Z. Swartz, Ralph A. Cacho, Naia Novy, Asim K. Bera, Alex Kang, Banumathi Sankaran, Matthew P. Johnson, Amala Phadkule, Mike Reppert, Damian Ekiert, Gira Bhabha, Lance Stewart, Justin R. Caram, Barry L. Stoddard, Elisabet Romero, C. Neil Hunter, David Baker

Nature Chemical Biology, Vol. 20, Issue 7, Pages 906-915

2024 July

Measuring the Total Photon Economy of Molecular Species through Fluorescent Optical Cycling (FOC)

Anthony Sica, Ash Hua, Belle Coffey, Kierstyn Anderson, Benjamin Nguyen, Alexander Spokoyny, Justin Caram

2024 January

Measuring the total photon economy of molecular species through fluorescent optical cycling

Anthony V. Sica, Ash Sueh Hua, Belle Coffey, Kierstyn P. Anderson, Lia A. Coffey, Benjamin T. Nguyen, Alexander M. Spokoyny, Justin R. Caram

Physical Chemistry Chemical Physics, Vol. 26, Issue 32, Pages 21850-21860

2024 January

Spectrally Selective Time-Resolved Emission through Fourier-Filtering (STEF)

Anthony V. Sica, Ash Sueh Hua, Helen H. Lin, Ellen M. Sletten, Timothy L. Atallah, Justin R. Caram

The Journal of Physical Chemistry Letters, Vol. 14, Issue 2, Pages 552-558

2023 January

Spectrally-selective Time-resolved Emission through Fourier-filtering (STEF)

Anthony Sica, Timothy Atallah, Justin Caram

2022 October

Decay-Associated Fourier Spectroscopy: Visible to Shortwave Infrared Time-Resolved Photoluminescence Spectra

Timothy L. Atallah, Anthony V. Sica, Ashley J. Shin, Hannah C. Friedman, Yaniv K. Kahrobai, Justin R. Caram

The Journal of Physical Chemistry A, Vol. 123, Issue 31, Pages 6792-6798

2019 August