Solution State CryoProbes
All of the spectrometers (500 MHz, 3 x 800 MHz, 2 x 900 MHz) for solution NMR applications are equipped with either 5 mm TXI/TCI Cryogenic probes. The lower temperature probes reduce the thermal noise and increase overall probe sensitivity. The higher proton sensitivity of cryogenic probes offers a four-fold signal enhancement in non-polar solvent and two-fold increase in aqueous samples compared to room temperature probe. This allows NMR experiments to be performed at very low concentrations in the ~10 micromolar range. In the TXI CryoProbe, the proton and deuterium circuits are in the cold state and in the TCI CryoProbe proton, deuterium and carbon are in the cold state. In the latter, the direct observe carbon sensitivity increases by a factor of two enabling improved performance of experiments that do not depend on proton detection. The 1.7 mm TCI MicroCryoProbe compatible with 800 MHz US2 systems has the ability to observe signal from very small volumes (30 μl). The incredibly high mass sensitivity of the probe makes it an ideal tool for applications which are sample limited. With the SampleJet the microprobe can be used in high throughput screening and metabolomics.
Spectrometer | CryoProbe | 2 mM Sucrose Anomeric S/N |
0.1% Ethylbenzene S/N |
0.1% CHCl3 Lineshape |
500 MHz | 5mm TXI | 470:1 | 4795:1 | 4.3/9.9/0.53 |
800 MHz | 5mm TXI | 766:1 | 7602:1 | 4.1/5.4/0.54 |
800 MHz US2 #1 | 5mm TCI | 830:1 | 7164:1 | 6.3/11.9/0.82 |
800 MHz US2 #2 | 5mm TCI | 930:1 | 9212:1 | 4.8/8.5/0.75 |
1.7mm TCI | 429:1 | 1958:1 | 12/24/1.12 | |
900 MHz | 5 mm TCI | 1035:1 | 8863:1 | 8.3/14.8/0.88 |
900 MHz US2 | 5 mm TCI | 956:1 | 9097:1 | 7.8/15.6/0.62 |
Summary of Solid State Probes
Probe | Make | Volume, ul | Spinning, KHz | X/Y Channel | Common Configurations |
600MHz DNP/89mm Wide Bore 4-Channel Avance III Console, Topspin 3.5 Software, 395GHz Gyrotron, LTMAS Cabinet |
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3.2mm HCN E-free RT | Bruker | 25 | 3-22 | 13C & 15N | HCN |
3.2mm HCN LT-DNP | Bruker | 25 | 3-13 | 13C & 15N | HCN |
1.9mm HCN LT-DNP | Bruker | 10 | 5-25 | 13C & 15N | HCN |
2.5mm HFXY LT-DNP | Phoenix | 18 | 5-25 | 23Na to 13C | HCN, HFCN, HPC |
750MHz/89mm Wide Bore 4-Channel NEO Console, Topspin 4 Software, BCU2 Cooling Unit |
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4mm HXY | Bruker | 80 | 3-15 | 23Na to 14N | HC, HCN |
4mm HFX | Bruker | 80 | 3-15 | 23Na to 49Ti | HC, FC, HFC |
3.2mm HCN E-free | Bruker | 25 | 3-22 | 13C & 15N | HCN |
1.9mm HXY | Bruker | 10 | 4-45 | 23Na to 14N | HC, HCN |
4mm HRMAS | Bruker | 60 | 3-15 | 13C & 15N | HCN |
900MHz/54mm Standard Bore 4-Channel Avance II Console, Topspin 3.2 Software, BCUX Cooling Unit |
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1.3mm HCN Fast MAS | Bruker | 2 | 30-65 | 13C & 15N | HC, HCN |
3.2mm HCN E-free | Bruker | 25 | 3-22 | 13C & 15N | HCN |
3.2mm HX | Bruker | 25 | 3-22 | 23Na to 49Ti | HX |
4mm HRMAS | Bruker | 60 | 3-15 | 13C & 15N | HCN, HCN |
1.2mm HFXY | Phoenix | 1.2 | 10 -60 | 23Na to 13C | HCN, HFCN, HFPC, HFCD |
1.6mm HFXY | Phoenix | 8 | 8-40 | 23Na to 13C | HCN, HFCN, HFPC, HFCD |