NMR Instrumentation

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Solution State CryoProbes

All of the spectrometers (500 MHz, 3 x 800 MHz, 2 x 900 MHz) for solution NMR applications are equipped with either 5 mm TXI/TCI Cryogenic probes. The lower temperature probes reduce the thermal noise and increase overall probe sensitivity. The higher proton sensitivity of cryogenic probes offers a four-fold signal enhancement in non-polar solvent and two-fold increase in aqueous samples compared to room temperature probe. This allows NMR experiments to be performed at very low concentrations in the ~10 micromolar range. In the TXI CryoProbe, the proton and deuterium circuits are in the cold state and in the TCI CryoProbe proton, deuterium and carbon are in the cold state. In the latter, the direct observe carbon sensitivity increases by a factor of two enabling improved performance of experiments that do not depend on proton detection. The 1.7 mm TCI MicroCryoProbe compatible with 800 MHz US2 systems has the ability to observe signal from very small volumes (30 μl). The incredibly high mass sensitivity of the probe makes it an ideal tool for applications which are sample limited. With the SampleJet the microprobe can be used in high throughput screening and metabolomics.

Spectrometer CryoProbe 2 mM Sucrose
Anomeric S/N
0.1%
Ethylbenzene S/N
0.1% CHCl3
Lineshape
500 MHz 5mm TXI 470:1 4795:1 4.3/9.9/0.53
800 MHz 5mm TXI 766:1 7602:1 4.1/5.4/0.54
800 MHz US2 #1 5mm TCI 830:1 7164:1 6.3/11.9/0.82
800 MHz US2 #2 5mm TCI 930:1 9212:1 4.8/8.5/0.75
1.7mm TCI 429:1 1958:1 12/24/1.12
900 MHz 5 mm TCI 1035:1 8863:1 8.3/14.8/0.88
900 MHz US2 5 mm TCI 956:1 9097:1 7.8/15.6/0.62

Summary of Solid State Probes

Probe Make Volume, ul Spinning, KHz X/Y Channel Common Configurations
600MHz DNP/89mm Wide Bore
4-Channel Avance III Console, Topspin 3.5 Software, 395GHz Gyrotron, LTMAS Cabinet
3.2mm HCN E-free RT Bruker 25 3-22 13C & 15N HCN
3.2mm HCN LT-DNP Bruker 25 3-13 13C & 15N HCN
1.9mm HCN LT-DNP Bruker 10 5-25 13C & 15N HCN
2.5mm HFXY LT-DNP Phoenix 18 5-25 23Na to 13C HCN, HFCN, HPC
750MHz/89mm Wide Bore
4-Channel NEO Console, Topspin 4 Software, BCU2 Cooling Unit
4mm HXY Bruker 80 3-15 23Na to 14N HC, HCN
4mm HFX Bruker 80 3-15 23Na to 49Ti HC, FC, HFC
3.2mm HCN E-free Bruker 25 3-22 13C & 15N HCN
1.9mm HXY Bruker 10 4-45 23Na to 14N HC, HCN
4mm HRMAS Bruker 60 3-15 13C & 15N HCN
900MHz/54mm Standard Bore
4-Channel Avance II Console, Topspin 3.2 Software, BCUX Cooling Unit
1.3mm HCN Fast MAS Bruker 2 30-65 13C & 15N HC, HCN
3.2mm HCN E-free Bruker 25 3-22 13C & 15N HCN
3.2mm HX Bruker 25 3-22 23Na to 49Ti HX
4mm HRMAS Bruker 60 3-15 13C & 15N HCN, HCN
1.2mm HFXY Phoenix 1.2 10 -60 23Na to 13C HCN, HFCN, HFPC, HFCD
1.6mm HFXY Phoenix 8 8-40 23Na to 13C HCN, HFCN, HFPC, HFCD