Services include:
- Structural elucidation of novel drug targets
- Optimizing antibodies and immunogens.
- Rapid construct design and production of purified soluble and membrane proteins for experimental purposes including high-throughput analysis and specialized assays
- High throughput NMR drug screening using an automated Bruker SampleJet
Rational drug design requires an understanding of the function and structure of proteins.
Cloning and Protein Production
- Bacterial, insect, and mammalian cell expression systems
- Unique methods for production of challenging proteins
Unique methods for overcoming poor expression and difficulties of purifying challenging protein targets.
Brian Kloss
Membrane Protein Optimization
- Detergent stability assays
- Purification in nanodiscs, bicelles and liposomes
- Small-scale cell-free assay and optimization
Protein Crystallization and Optimization
- Automated high throughput crystallization screening
- Crystal optimization guided by X-ray diffraction
Jonah Cheung
Michael Rudolph
High Resolution Structure Determination
- Dedicated NYSBC-operated synchrotron beamline (NYX) at NSLS II
- All standard phasing techniques
Applications
- Enzyme-inhibitor complexes to guide drug design
- Multi-protein membrane complexes
- Structure-guided antibody design and high-resolution epitope mapping
- Elucidating molecular mechanisms of human disease at the atomic level
- Purified proteins for assay development and small molecule library screening
- Testing and validating AlphaFold hypotheses and structures
The Research Service Laboratory works with academic and for-profit scientists on a fee-for-service basis. It also has funding provided by the NIH and other federal and private granting agencies.
Please contact:
Willa Appel, PhD (Executive Director)
appelw@nysbc.org
(212) 939-0660 ext. 112
The Laboratory operates dedicated equipment for rapid protein purification, biophysical assays, NMR, X-ray and electron microscopy structural analysis and drug screening.
A combination of unique technologies allows the group to manage multiple research projects in parallel at a rapid pace