High-Throughput Cloning and Expression
- Biomek Liquid Handler
- 96-deep well block expression
- Robotic sonicator
- DodecaTM system for high-throughput protein gels
Rapid Protein Purification
Four automated GE Life Sciences AKTAxpress chromatography systems allows the group to purify up to 16 unique proteins, in parallel overnight, using two-column chromatography methods. The group also has access to two additional fully-customizable FPLC systems for improved optimization of purification.
Protein Stability and Biophysical Assays
- Protein quality and stability using thermal denaturation (Tycho)
- Binding assay by microscale thermophoresis (MST)
- Electrophysiology for transporter, pumps and channels by solid-supported membrane (SSM) technology (SURFER)
- Lipid bilayer recording (Orbit)
An Integrated Crystallization Pipeline
Integrated robotic technologies controlled from a central server allows the Research Services Laboratory to screen, optimize, and monitor crystallization of purified proteins with ease. Shown above from left to right are the Formulatrix NT8 drop-setting robot, the Formulator liquid handling robot, and a Rock Imager 1000 automatic crystal imager. Separate imagers set to 22°C and 4°C allow for greater coverage of crystallization space.
Rapid X-ray Data Collection
The Research Services Laboratory has routine rapid access to the NYSBCʼs state-of-the art NYX synchrotron beamline at National Synchrotron Light Source II at Brookhaven National Labs, NY.