NYX Beamline

The X-Ray Department has constructed a macromolecular crystallography undulator beamline (NYX) at the National Synchrotron Light Source II which began user operations in 2019. The beamline will allow smaller crystal size, higher energy resolution, and a greater degree of automation than NYSBC’s previous beamlines (X4A and X4C at NSLS). The improved instrumentation, combined with the 10,000-fold increase in X-ray brightness possible at NSLS-II, will greatly expand our ability to determine protein structures, making experiments that were previously impossible seem almost routine.

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Capabilities

  • High energy resolution down to ΔE/E = 5 x 10-5 without loss of flux. Capable of producing sharp absorption edges for challenging de novo structure solution by MAD/SAD phasing.
  • Beam size: 30 um (v) x 50-100 um (h); focused full-beam flux >1012photons/sec. Capable of using micron-sized samples, in particular for macromolecular complexes and membrane proteins.
  • X-ray energy coverage from 7-17.5 keV. Capable of anomalous diffraction experiments from iron K (7.1 keV) to uranium LIII (17.1 keV) edge, covering the most commonly used heavy atoms for MAD/SAD phasing. Enhanced anomalous signals for native SAD phasing from remote K resonance for light atoms including P, S and Cl.
  • High-throughput data collection with a custom ADSC pixel array detector and a cryogenic sample changer controlled with Blu-Ice data collection software.
  • Remote access using NX client.
  • UniPucks (preferred) or ALS pucks with SPINE or ALS bases and any loop style can be used. The dewar has a capacity of 16 pucks.

In-house Facility

NYSBC maintains a modern rotating-anode X-ray source at its main facility in Manhattan, equipped with fast CCD X-ray detectors, cryosystems, and computational resources. This facility is used by NYSBC staff to support collaborative research projects, as a pre-screening step before taking crystals to NYSBC’s beamline, and for direct X-ray data collection. Many of the crystal structures solved by NYSBC staff in the last few years relied exclusively on this system.