In collaboration with scientists and equipment at NYSBC’s National Synchrotron Light Source beamline (X4), researchers from the University of Pennsylvania, the Children’s Hospital of Philadelphia and Duke University used X-ray crystallography to study the mechanisms leading to heparin-induced thrombocytopenia (HIT). Their findings, which provide a basis to develop new diagnostics and therapeutics for HIT, were [learn more]

RNA-directed RNA polymerase (RdRp) is encoded in the genome of all RNA-containing viruses (except retroviruses), such as the polio, hepatitis A, C and E, measles, rabies, Dengue, West Nile, influenza and many others viruses. It is an essential protein that catalyzes the replication of RNA from an RNA template and its sequence is among the [learn more]

In eukaryotic cells, DNA is stored in the nucleus, separated from the rest of the cell by a double membrane called the nuclear envelope. Yet, many large molecules such as proteins and RNA must cross this barrier to carry out gene expression and other critical activities. They do so via regulated transport through nuclear pore [learn more]

The Special Projects Group at NYSBC has built a pipeline to clone, express, purify, and determine the structures of proteins from Coxiella burnetii, a highly infectious pathogen that can cause disease in humans and other mammals. In under ten months, this group analyzed all 1847 protein-coding genes of C. burnetii, cloned 500 of these genes,  [learn more]

Staphylococcal enterotoxin B (SEB) is a superantigen that can simultaneously bind to MHC proteins on the surface of most cells and TCR receptors expressed by immune T cells. This interaction activates a large number of T cells, causing high fevers and potentially death. Neutralizing antibodies are currently the only therapeutic option and must be optimized  [learn more]

High-resolution structure could lead to design of more effective drugs with fewer side effects; biochemical assays uncover possible role in degrading destructive oxygen compounds Valium, one of the best known antianxiety drugs, produces its calming effects by binding with a particular protein in the brain. But the drug has an almost equally strong affinity for [learn more]

Rapid structural and biochemical characterization, by NYSBC researchers, of two recurring PKA mutants found in two distinct cancers will aid in the design of new treatments. Protein kinase A (PKA) is a cAMP-dependent kinase involved in the regulation of a number of important cell processes including metabolism, proliferation, and gene expression. In the past year, [learn more]

Phospholipids are the major constituents of the cell membrane, the water-impermeable barrier that surrounds each and every cell, crucial for its existence, wellbeing and survival. All cells, prokaryotic and eukaryotic alike, need to be able to assemble their membranes, and synthesize their components. The key step in phospholipid biosynthesis involves the reaction of a diacylglycerol [learn more]

X-ray Crystal Structures of Ricin Toxin in Complex with Neutralizing and Non-neutralizing antibodies In collaboration with researchers at the Wadsworth Center, NYSBC crystallographers solved the crystal structures of the ricin enzymatic subunit in complex with the antigen binding domain of several single-chain monoclonal antibodies. Ricin is a well-known biothreat agent that has been the focus [learn more]

Structure of membrane protein that plays a role in signaling cell death could be new target for anticancer drugs Sometimes a cell has to die—when it’s done with its job or inflicted with injury that could otherwise harm an organism. Conversely, cells that refuse to die when expected can lead to cancer. So scientists interested [learn more]