NMR Department

NMRNMR – From protein to structure We have the unique ability to probe atomic level details of molecules using both Solution and Solid State NMR techniques at a range of field strengths (500-900 MHz). The ultra high field magnets facilitate the study of complex structures and dynamics of some of the largest macromolecules at highest sensitivity and atomic resolution afforded by current technology.

About the NMR Department

The NMR facility at NYSBC is a shared resource that tackles challenging problems in structural biology and materials science. The facility is supported by experienced staff dedicated to providing hands-on-training in data collection and available for consultation on individual projects.

Highlights
  • Access to six high field magnets (800-900 MHz) with solution and solid state NMR capabilities.
  • Applications of Dynamic Nuclear polarization in the study of biomolecules and materials science.
  • Staff expertise in structural and dynamic characterization of high molecular weight proteins, nucleic acids and complexes.
  • Pulse sequence development for specific applications in solution and solid state NMR.
  • Study of molecular dynamics by NMR and simulations.
  • Sample Jet with automation for metabolomics and high throughput drug screening.
  • Hands on training.
  • Workshops on data processing and analysis.
  • Theory course on NMR spin physics offered with Columbia University.
NMR Applications
  • Chemical shift assignment of large proteins and nucleic acids.
  • Chemical shift perturbation assays to probe binding.
  • Structure determination of proteins, nucleic acids and complexes.
  • In cell NMR
  • High throughput screening of small molecules in drug discovery.
  • Metabolomics
  • Nuclear relaxation as a probe of biomolecular dynamics.
  • Combining NMR data with SAXS/SANS and CryoEM to develop model structures of supra-molecular assemblies.
  • Membrane protein structures in solution and micro-crystalline form by solid state NMR.