SEMC FORUM on Thursday, June 20, 2019:  

Weighing Molecules with Light – a new way to study biomolecules

Matthias Langhorst Refeyn

12pm – NYSBC Main Conference Room

The principles behind mass photometry, a technology based on interferometric scattering microscopy: It represents a truly novel approach capable of analyzing individual biomolecules in solution by measuring the mass. Given that most physiological and pathological processes rely on the interaction between different biomolecules, an approach that cannot only observe these species in a universal, label-free fashion, but also measure their mass accurately and with molecular resolution, could be transformative for our ability to characterize the composition, structure and dynamics of biomolecules. Applications include accurate determination of the degree of heterogeneity of biomolecule samples, quantification of molecular interaction strengths and kinetics, optimizing conditions for sample stability and interactions, studies of self-assembly processes as well as precise characterization of molecular concentration. I will illustrate the unique capabilities of mass photometry by discussing a broad selection of recent examples and provide insight into the strengths and limitations of the approach.

 

SEMC FORUM on Monday,  June 24, 2019:  

Unique Capabilities of the DE-64 for Cryo-EM

Benjamin Bammes, Ph.D.

Direct Electron LP, San Diego, CA

12pm – NYSBC Main Conference Room

Over the past five years, electron cryo-microscopy (cryo-EM) has become the most popular and effective technique in structural biology, due to the hardware and software innovations that delivered cryo-EM’s “resolution revolution.” However, there are still significant opportunities for further improvement, especially with respect to enabling new techniques and maximizing data quality and throughput of existing techniques in order to satisfy the high demand for cryo-EM resources and alleviate the high cost of purchasing, maintaining, and operating cryo-EM equipment.

Direct Electron’s DE-64 is a versatile direct detection camera for cryo-EM. In this talk, I will share single-particle cryo-EM results from this camera and discuss its unique technologies for reducing image noise and maximizing signal-to-noise ratio (SNR). I will discuss how the DE-64 enables very-high-throughput continuous-tilt tomography and microED (electron diffraction) at sub-Angstrom resolution.